AA-Dutp-Cy3 a Novel Fluorescent Labeling Agent for Nucleotides
نویسندگان
چکیده
منابع مشابه
Cyanine dye dUTP analogs for enzymatic labeling of DNA probes.
Fluorescence in situ hybridization (FISH) has become and indispensable tool in a variety of areas of research and clinical diagnostics. Many applications demand an approach for simultaneous detection of multiple target sequences that is rapid and simple, yet sensitive. In this work, we describe the synthesis of two new cyanine dye-labeled dUTP analogs, Cy3-dUTP and Cy5-dUTP. They are efficient ...
متن کاملFluorescent dUTP helps characterize 10 novel tetranucleotide microsatellites from an enriched salamander
Ten tetranucleotide microsatellite loci were isolated and characterized from Indiana (USA) populations of the smallmouth salamander, Ambystoma texanum . As opposed to individually labelling primers that were not yet known to be polymorphic, we used fluorescent dUTP to assess genetic variability and found it to be very effective. Allelic diversity ranged from two to 18 alleles per locus and obse...
متن کاملAnalysis of loss of heterozygosity in microdissected tumor cells from cervical carcinoma using fluorescent dUTP labeling of PCR products.
The use of fluorescently labeled nucleotides (F-dUTP) in the PCR for genetic typing of microsatellite polymorphisms was investigated. Microdissected tumor cells from cervical squamous cancer biopsies were compared with cells from surrounding normal tissue for loss of heterozygosity (LOH) at the mismatch repair gene hMLH1. Removal of unincorporated F-dUTP before analysis is necessary to reduce b...
متن کاملIndium-111 maltolate complex as a cell labeling agent for SPECT imaging
Introduction: Due to the cell labeling potentials of various metal-maltol complexes, the idea of developing a possible cell labeling imaging agent using single photon emission computed chromatography (SPECT) with[111In]-maltolate complex has been investigated. Methods: [111In] labeled 3-hydroxy-2-methyl-4H-pyran-4-onate ([111In]-maltolate) was prepared using f...
متن کاملA fluorescent photochromic compound for labeling biomolecules.
A fluorescent photochromic compound, composed of diarylethene, fluorescein and succinimidyl ester units, was developed for the controllable fluorescent labeling of biomolecules based on a small molecule.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemistry & Pharmacology: Open Access
سال: 2014
ISSN: 2167-0501
DOI: 10.4172/2167-0501.1000137